Induced pluripotent stem cells (iPSCs) are generated through a gradual process in which somatic cells undergo a number of\nstochastic events. In this study, we examined whether two different doxycycline-inducible iPSCs, slow-forming 4F2A-iPSCs and\nfast-forming NGFP-iPSCs, have equivalent levels of pluripotency. Multiplex reverse-transcriptase PCR generated gene expression\nprofiles (GEPs) of 13 pluripotency genes in single initially formed-iPSC (if-iPSC) colonies of NGFP and 4F2A group. Assessment\nof GEP difference using a weighted root mean square deviation (wRMSD) indicates that 4F2A if-iPSCs are more closely related\nto mESCs than NGFP if-iPSCs. Consistently, Nanog and Sox2 genes were more frequently derepressed in 4F2A if-iPSC group.We\nfurther examined 20 genes that are implicated in reprogramming. They were, overall, more highly expressed in NGFP if-iPSCs,\ndiffering from the pluripotency genes being more expressed in 4F2A if-iPSCs. wRMSD analysis for these reprogramming-related\ngenes confirmed that the 4F2A if-iPSC colonies were less deviated from mESCs than the NGFP if-iPSC colonies. Our findings\nsuggest that more important in attaining a better reprogramming is the mode of action by the given reprogramming factors, rather\nthan the total activity of them exerting to the cells, as the thin-but-long-lasting mode of action in 4F2A if-iPSCs is shown to be\nmore effective than its full-but-short-lasting mode in NGFP if-iPSCs.
Loading....